active rhoa -gtp [26904]
[30 µl]
catalog number: 26904
gene symbol: rhoa
description: anti-active rhoa mouse monoclonal antibody
background: small gtpases are a super-family of cellular signaling regulators. rhoa belongs to the rho sub-family of gtpases. rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. it regulates the formation of stress fibers and focal adhesions in fibroblasts and ca 2+ sensitivity of smooth muscle contraction.
immunogen: recombinant full length protein of active rhoa
applications: ip, ihc
recommended dilutions: 1 µg for 1~2 mg total cellular proteins for ip;
1:100 dilution for ihc
concentration: 1 mg/ml
host: mouse
format: liquid
clonality: monoclonal
isotype: igg2b
purity: purified from ascites
storage buffer:
preservative: no
constituents: pbs (without mg 2+ and ca 2+ ), ph 7.4, 150 mm nacl, 50% glycerol
species reactivity: anti-active rhoa antibody recognizes active rhoa from vertebrates.
storage conditions: store at -20°c. avoid freeze / thaw cycles
immunoprecipitation/western blot:
ip/wb analysis of active rhoa protein. purified recombinant rhoa proteins were loaded with gdp (lane 1) or gtpγs (lane 2). these proteins were immunoprecipitated with anti-active rhoa mouse monoclonal antibody (cat. # 26904). after sds/page, the membrane filter was probed with anti-rhoa mouse monoclonal antibody (cat. # 26007).
publications:
1.
j neurochem. 2011 dec;119(6):1306-16
2.
oncogene. 2012 aug 30;31(35):3913-23
3.
carcinogenesis. 2010 jul;31(7):1230-7
4.
development, growth & differentiation. volume 55, issue 4, pages 579–590, may 2013
5.
cell death and differentiation (2013) 20, 1031–1042
6.
j biol chem. 2013 jul 12;288(28):20217-27
7.
diabetes. 2013 nov;62(11):3817-27
8.
journal of neurochemistry volume 127, issue 2, pages 152–162, october 2013
9.
diabetes. 2014 feb;63(2):701-14
10.
cell death and disease (2013) 4, e848
11.
biochimica et biophysica acta (bba) - general subjects volume 1840, issue 8, august 2014, pages 2482–2490
12.
j cell sci. 2014 mar 15;127(pt 6):1229-41
13.
cytokine volume 69, issue 1, september 2014, pages 47–55
14.
plos one. 2014 oct 16;9(10):e110851
武汉纽斯特生物技术有限公司
18972339958
中国 武汉